Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene

Kamarudin, Kamarul Rahim and Mohamed Rehan, Maryam and Mohamed Rehan, Aisyah (2018) Species identification and molecular phylogenetics of processed sea cucumbers from Malaysian market based on 12s mitochondrial rRNA gene. Tropical Agricultural Science, 41 (4). pp. 1833-1851. ISSN 2231-8542

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Abstract

Extensive processing of sea cucumber causes body deformation of the marine organism, hence causing difficulties in species identification of processed sea cucumbers. Due to the copresence of cases of unlabelled or mislabelled sea cucumber products in Malaysian markets and worldwide, a study was conducted to determine the species identities of processed sea cucumbers from selected Malaysian markets using non-protein-coding 12S mitochondrial rRNA gene. Phylogenetic analyses based on the distance-based Neighbour Joining method, and the character-based methods i.e. the Maximum Parsimony method, Maximum Likelihood method, and the Bayesian Analysis method of 81 ingroup sequences representing 63 processed sea cucumber specimens, 13 fresh and processed reference samples for species identification, and five fresh additional specimens from Teluk Nipah Beach, Pangkor Archipelago and Manukan Island, Sabah suggested the presence of three main clusters i.e. a gamat family cluster consisting of family Stichopodidae (Stichopus horrens, Stichopus vastus, and Thelenota anax) and two clusters of timun laut family comprising family Holothuriidae (Holothuria (Mertensiothuria) leucospilota, Holothuria (Metriatyla)scabra, Holothuria (Metriatyla) lessoni, Holothuria (Halodeima) atra, and Holothuria (Halodeima) edulis) and family Caudinidae (Acaudina molpadioides). The outcomes of this study also highlighted the availability of 40 new 12S mitochondrial rRNA gene sequences deposited in the GenBank that can be utilised by the enforcement agencies to monitor and overcome the issues of species substitution and product mislabelling of processed sea cucumber products in Malaysian markets.

Item Type:	Article
Uncontrolled Keywords:	12S rRNA gene; gamat, Malaysian markets; phylogenetic analysis; processed sea cucumber; timun laut
Subjects:	H Social Sciences > HF Commerce > HF5001-6182 Business
Divisions:	Faculty of Applied Science and Technology > Department of Technology and Natural Resources
Depositing User:	UiTM Student Praktikal
Date Deposited:	25 Jan 2022 00:23
Last Modified:	25 Jan 2022 00:23
URI:	http://eprints.uthm.edu.my/id/eprint/5919

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