A noninvasive molecular approach: exploiting species-locus-specific PCR primers in defeating numts and DNA cross-contamination of cercopithecidae

Abu Bakar, Muhammad Abdul Latiff and Aifat, N. R. and Yaakop, S. and Md Zain, B. M. (2017) A noninvasive molecular approach: exploiting species-locus-specific PCR primers in defeating numts and DNA cross-contamination of cercopithecidae. The Journal of Animal & Plant Sciences, 27 (3). pp. 1015-1053. ISSN 1018-7081

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Abstract

The lack of a standardized, noninvasive molecular approach to studying genetic aspects of primates has made it hard for primatologists to decode the evolutionary history of these species. Researchers must optimize their own techniques to fully exploit the available samples. Lack of species-locus-specific primers also contributes to difficulties in using noninvasive genetic samples. Thus, the objectives of this study were to develop a standardized technique to collecting samples noninvasively, propose newly designed species-locus-specific primers, and optimize conditions for polymerase chain reaction (PCR) for Macaca fascicularis, M. nemestrina, Trachypithecus cristatus, and T. obscurus. Nine new species-locus-specific primers for three different loci of mitochondrial DNA, namely D-loop, cytochrome oxidase subunit I (COI), and cytochrome b, were successfully designed. These primers proved to be efficient in amplifying larger datasets (up to ~1,000 bp) of the targeted species in the optimized PCR conditions. The species-locus-specific primers are able to anneal to host DNA alone in highly contaminated feces of highlighted species. They can also offer alternatives measures in avoiding contamination related to nuclear insertion of mitochondrial pseudogenes (numts).

Item Type:	Article
Uncontrolled Keywords:	Noninvasive; Species-locus-specific primers; Numts; Macaca; Trachypithecus
Subjects:	T Technology > TP Chemical technology
Divisions:	Faculty of Applied Science and Technology > Department of Technology and Natural Resources
Depositing User:	Mrs. Siti Noraida Miskan
Date Deposited:	07 Dec 2021 06:50
Last Modified:	07 Dec 2021 06:50
URI:	http://eprints.uthm.edu.my/id/eprint/4545

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