Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia

Abu Bakar, Mohd Fadzelly and Abdul Karim, Fifilyana and Suleiman, Monica and Isha, Azizul (2015) Phytochemical constituents, antioxidant and antiproliferative properties of a Liverwort, Lepidozia borneensis Stephani from Mount Kinabalu, Sabah, Malaysia. Evidence-Based Complementary and Alternative Medicine. pp. 1-9. ISSN 1741-427X

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Abstract

The study aimed to investigate the phytochemical contents, antioxidant and antiproliferative activity of 80% methanol extract of Lepidozia borneensis. The total phenolic and total flavonoid contents were analysed using Folin-Ciocalteu and aluminium chloride colorimetric methods. Antioxidant properties were evaluated by using FRAP, ABTS, and DPPH assays while the effects of L. borneensis on the proliferation of MCF-7 cell line were evaluated by using MTT assay. The results showed that the total phenolic and flavonoid contents were 12.42 ± 0.47 mg GAE/g and 9.36 ± 1.29 mg CE/g, respectively. The GC-MS analysis revealed the presence of at least 35 compounds. The extract was found to induce cytotoxicity against MCF-7 cell line with IC50 value of 47.33 ± 7.37 µg/mL. Cell cycle analysis showed that the extract induced significant arrest at G0/G1 at 24 hours of treatment. After 72 hours of treatment, the proportion of cells in G0/G1 and G2-M phases had decreased significantly as compared to their control. Apoptosis occurred during the first 24 hours and significantly increased to 30.8% after 72 hours of treatment. No activation of caspase 3 was observed. These findings suggest that L. borneensis extract has the potential as natural antioxidant and anticancer agents.

Item Type: Article
Uncontrolled Keywords: nil
Subjects: Q Science > QK Botany
T Technology > T Technology (General)
Divisions: Faculty of Applied Science and Technology > Department of Technology and Natural Resources
Depositing User: Mrs. Mashairani Ismail
Date Deposited: 21 Dec 2021 07:11
Last Modified: 21 Dec 2021 07:11
URI: http://eprints.uthm.edu.my/id/eprint/4831

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